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KMID : 0379119880160040214
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Korean Journal of Mycology
1988 Volume.16 No. 4 p.214 ~ p.219
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Studies on Protoplast Formation and Reversion of Pleurotus sapidus Kalchbr
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Abstract
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Factors affecting protoplast formation and reversion were investigated in Pleurotus sapidus kalchbr. For release of protoplast, enzyme mixture of Novozyme 234, β-D-glucanase and β-glucuronidaae was most effective, when mycelium of 0.6 M sucrose solution as osmotic stabilizer without addition of buffer solution. The yield of protoplast was highest with mycelium cultured for 4 days on mushroom complete agar medium at 30℃.
Protoplasts of Pleurotus sapidus were reverted to normal hyphal growth with maximum reversion frequency of 2% on Mushroom complete agar medium stabilized with 0.6 M sucrose solution and covered by 0.75 agar layer.
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